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| Part No. | GB02009 | |||
| CAS NO: | 5625-37-6 | |||
| EINECS NO | 227-057-6 | |||
| Ingredients | Piperazine-N,N'-bis(2-ethanesulfonic) acid | |||
| Content of active ingredients | ≥99.5% | |||
| Grade | AR/ACS equivalent | |||
| Molecular Formula | C8H18N2O6S2 | |||
| Molecular Weight : | 302.37 | |||
| Buffering Range | 6.1~7.5 | |||
| Brand | Neocide® | |||
| Packaging | Pkg of 1Kg/25Kg | |||
| Storage | Room Temperature | |||
| Items | Specification | |||
| Apparence | White Crystalline Powder | |||
| APHA (Color, 0.5M NaOH solution) | ≤20 | |||
| Solubility (Turbidity,0.5M NaOH solution) | Clear | |||
| Assay (Titration by HCI) | ≥99.5% | |||
| Infrared Spectrum Complies | Conforms to Structure | |||
| UV Absorbance 260mm 0.1M aqueous solution | absorbance≤0.05 | |||
| UV Absorbance 280mm 0.1M aqueous solution | absorbance≤0.05 | |||
| UV Absorbance 260mm 0.5M aqueous solution | absorbance≤0.10 | |||
| UV Absorbance 290mm 0.5M aqueous solution | absorbance≤0.10 | |||
| Loss on drying(105℃ for 3 Hours) Wt% | ≤0.5% | |||
| Sulfated Ash （650℃ for 3 Hours）Wt% | ≤0.1% | |||
| Heavy Metals Pb | ≤5ppm | |||
| Iron Fe | ≤5ppm | |||
| Calcium Ca | ≤10ppm | |||
| Usage | ||||
Pipes Free Acid is a very important biological buffering agent that can be used for the purification of microtubule proteins using phosphoric acid cellulose chromatography The gel filtration method was used to purify recombinant GTP-binding proteins ARF1 and ARF2 as a buffer for crystallization of ketone reductase from Escherichia coli; due to It can form free radicals, so it is not suitable for application in redox systems; in cation exchange chromatography, low concentrations should be used Buffer solution, because it has a relatively high ion strength and its pKa value is concentration-dependent. PIPES Free acid buffer can also be used for flow cytometry to detect the cell cycle. | ||||
| Part No. | GB02009 | |||
| CAS NO: | 5625-37-6 | |||
| EINECS NO | 227-057-6 | |||
| Ingredients | Piperazine-N,N'-bis(2-ethanesulfonic) acid | |||
| Content of active ingredients | ≥99.5% | |||
| Grade | AR/ACS equivalent | |||
| Molecular Formula | C8H18N2O6S2 | |||
| Molecular Weight : | 302.37 | |||
| Buffering Range | 6.1~7.5 | |||
| Brand | Neocide® | |||
| Packaging | Pkg of 1Kg/25Kg | |||
| Storage | Room Temperature | |||
| Items | Specification | |||
| Apparence | White Crystalline Powder | |||
| APHA (Color, 0.5M NaOH solution) | ≤20 | |||
| Solubility (Turbidity,0.5M NaOH solution) | Clear | |||
| Assay (Titration by HCI) | ≥99.5% | |||
| Infrared Spectrum Complies | Conforms to Structure | |||
| UV Absorbance 260mm 0.1M aqueous solution | absorbance≤0.05 | |||
| UV Absorbance 280mm 0.1M aqueous solution | absorbance≤0.05 | |||
| UV Absorbance 260mm 0.5M aqueous solution | absorbance≤0.10 | |||
| UV Absorbance 290mm 0.5M aqueous solution | absorbance≤0.10 | |||
| Loss on drying(105℃ for 3 Hours) Wt% | ≤0.5% | |||
| Sulfated Ash （650℃ for 3 Hours）Wt% | ≤0.1% | |||
| Heavy Metals Pb | ≤5ppm | |||
| Iron Fe | ≤5ppm | |||
| Calcium Ca | ≤10ppm | |||
| Usage | ||||
Pipes Free Acid is a very important biological buffering agent that can be used for the purification of microtubule proteins using phosphoric acid cellulose chromatography The gel filtration method was used to purify recombinant GTP-binding proteins ARF1 and ARF2 as a buffer for crystallization of ketone reductase from Escherichia coli; due to It can form free radicals, so it is not suitable for application in redox systems; in cation exchange chromatography, low concentrations should be used Buffer solution, because it has a relatively high ion strength and its pKa value is concentration-dependent. PIPES Free acid buffer can also be used for flow cytometry to detect the cell cycle. | ||||
