Recommended Co-Application Solution for Tris and Tris-HCl Buffer System
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Recommended Co-Application Solution for Tris and Tris-HCl Buffer System

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Recommended Co-Application Solution for Tris and Tris-HCl Buffer System

Tris (Tris(hydroxymethyl)aminomethane) and Tris-HCl (Tris hydrochloride), as commonly used buffer pairs, provide a convenient, stable, and suitable pH environment for IVD reagents. For greater ease of use, we highly recommend the co-application solution of NeoCide® Tris and NeoCide® Tris-HCl produced by Suzhou Genemill.

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I. Principle of Co-Application

Tris is a weak base with a pKa of 8.1 at room temperature (25°C), offering an effective buffering range of pH 7.0–9.1. Tris-HCl is the hydrochloride salt derived from Tris.

  • Aqueous solutions prepared from Tris solids are alkaline (pH 10.0–11.5).

  • Aqueous solutions prepared from Tris-HCl solids are acidic (pH 4.2–5.0).

By blending high-purity Tris and Tris-HCl solids at specific ratios, customized buffer solutions with target pH values can be flexibly achieved.

II. Co-Application Protocol

Target pH and concentration can be attained by adding high-purity Tris and Tris-HCl at precise mass or molar ratios. Refer to the attached table for detailed data (e.g., mass/molar ratios and dosages for 0.01M Tris buffer at various pH levels).

III. Advantages

  • Eliminates the need for strong acids/bases (e.g., HCl or NaOH), avoiding restrictions on handling, procurement, and storage.

  • Streamlines workflows for experiments and production.

IV. NeoCide® Tris & Tris-HCl by Suzhou Genemill

  1. Purity: ≥99.5% for both products.

  2. Low impurities: Strict control of metal ions and other contaminants.

  3. Quality compliance: ISO 13485:2016 certified with rigorous batch-to-batch consistency.

  4. COA: Detailed quality specifications are provided in the attached Certificates of Analysis (COA).

  5. Packaging: Plastic drums for convenient use in production facilities.


Recommendation: To ensure buffer quality and experimental reliability, always use high-purity Tris/Tris-HCl reagents to avoid interference from impurities.

For technical support or inquiries, please contact us. We are committed to supporting your research and production success!

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COMPANY PROFILE
Specialized in developing, producing, and selling raw materials for IVD reagents.
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  Email:aimeegao@genemill.com
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