How To Select A Preservative for Immunological Reagents: Suzhou Genemill NeoCide PC-300 Antimicrobial Agent?
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How To Select A Preservative for Immunological Reagents: Suzhou Genemill NeoCide PC-300 Antimicrobial Agent?

Views: 2     Author: Site Editor     Publish Time: 2024-03-05      Origin: Site

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This article summarizes the usage of Proclin 300 based on 25 papers on immunological reagents. It aims to provide helpful insights and guidance for using antimicrobial agents in relevant assays.


  1. "Determination of Estradiol in Human Urine by Antigen-Coated Microplate Chemiluminescent Enzyme Immunoassay"
    Domestic BSA blocking solution used was a 50 mmol/L phosphate-buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Proclin-300. The E3 standard solution matrix consisted of a 50 mmol/L PBS buffer (pH 7.4) containing 2% BSA, 0.5% hydrolyzed gelatin, and 0.1% Proclin-300. The washing solution used was a PBST buffer solution (10 mmol/L PBS, pH 7.4) containing 0.05% Tween-20.

  2. "Comparative Analysis of Sandwich and Competitive Chemiluminescent Immunoassays for Detecting Hyaluronic Acid in Human Serum"
    For the preparation of calibration samples, a dilution solution containing 3% bovine serum albumin (BSA) and 1% casein, along with 5‰ Proclin-300, in 0.01 mol/L PBS (pH 7.4), was used as the dilution solution for the calibration samples.

  3. "Preparation, Storage, and Application of Immunomagnetic Beads for Detection of Escherichia coli O157:H7"
    Among them, Formula 2 (bovine serum albumin 15.0 g/L, casein 10.0 g/L, trehalose 10.0 g/L, PVP 2.0 g/L, ascorbic acid 5.0 g/L, ProClin 300 2.5 g/L) showed the best storage efficacy.

  4. "Establishment of a Biotin-Streptavidin Enzyme-Linked Immunosorbent Assay for Procalcitonin"
    Recombinant PCT protein was prepared as a series of reference standard solutions at concentrations of 0.5, 2.0, 5.0, 10.0, and 20.0 ng/mL using a buffer solution containing 1% BSA, 0.05% ProClin 300, and 50 nmol/L pH 7.2 Tris-HCl.

  5. "Competitive Chemiluminescent Enzyme Immunoassay for Testosterone Detection"
    ProClin 300 (1:2000) was added to each solution as a preservative.

  6. "Analysis and Countermeasures for Abnormal Results in Enzyme-Linked Immunosorbent Assay"
    In the enzyme immunoassay, sodium azide is prohibited for use as a preservative in the samples. Instead, alternative preservatives such as ProClin or thimerosal can be used.

  7. "Enzyme-Linked Immunosorbent Assay for the Detection of PAF Receptor RNA by Polymerase Chain Reaction Product." A 0.01 mol/L carbonate buffer solution with a pH of 7.4, containing 30 g/L bovine serum albumin and 0.1% preservative Proclin 300.

  8. "Establishment of a Chemiluminescent Immunoassay Method for Human B-hCG." Blocking solution (pH 7.9 PBS, 1% BSA, 0.25% Proclin-300); Preparation of alkaline phosphatase conjugate: Equal amounts of glycerol and 0.1% Proclin-300 were added to the product and stored at 4°C.

  9. "Clinical Application of a Rapid Detection and Typing Method for Human Papillomavirus." Colloidal gold dilution solution (20 mmol/L PB, 150 mmol/L NaCl, 1% BSA, 0.1% Triton X-100, 2% sucrose, 0.01% Proclin 300).

  10. "Preparation and Identification of Monoclonal Antibodies against Human Cytokeratin 19 Fragments." A 0.01 mol/L PBS (pH 7.4) containing 1% casein and 0.5% Proclin-300 was used as the diluent.

  11. "Development of ELISA Test Kit for Anti-Serum Glutamate Decarboxylase Antibodies." The enzyme-labeled secondary antibody was diluted in 25% bovine serum PBS buffer (pH 7.4) at ratios of 1:5,000, 1:10,000, and 1:20,000. It was then mixed with 1:3,000 Proclin-300 preservative and stored in a light-protected manner at 4°C.

  12. "Establishment and Application of a Chemiluminescent Immunoassay Method for Quantitative Determination of Hepatitis B Surface Antibody in Human Serum." A dilution solution of newborn calf serum containing 0.1% Proclin-300 as a preservative was used as the calibrator.

  13. "Establishment of a Neuron-Specific Alcohol Dehydrogenase Photoluminescence Immunoassay." Standard buffer solution (50 mmol/L Tris-HCl, 1.5% BSA, 0.9% NaCl, 0.05% Proclin-300, 0.01% Tween-20, pH 7.8).

  14. "Establishment of a Chemiluminescent Quantitative Immunoassay Method for Carbohydrate Antigen CA72-4." Both the calibrator and enzyme conjugate dilution solution were prepared in a 50 mmol/L pH 7.4 PB buffer containing 20% bovine serum and 0.1% Proclin 300. The blocking solution consisted of a 20 mmol/L pH 7.4 PB buffer containing 10% sucrose, 5% BSA, and 0.1% Proclin 300.

  15. "Chemiluminescent Enzyme Immunoassay for the Detection of Carcinoembryonic Antigen in Human Serum Using a Microplate." Blocking solution: 0.05 mol/L phosphate buffer (PBS, pH 7.4) containing 1% BSA and 0.1% Proclin-300.

  16. "Clinical Determination of Progesterone in Human Serum Using a Microplate Chemiluminescent Enzyme Immunoassay." Blocking solution: 0.05 mol/L phosphate buffer (PBS, pH 7.4) containing 1% BSA and 0.1% Proclin-300. Assay buffer: 0.05 mol/L PBS (pH 7.4) containing 1% BSA, 0.8% hydrolyzed gelatin, and 0.1% Proclin-300. Purification of anti-rabbit IgG using donkey anti-rabbit IgG, adding 0.05% Proclin-300 as a biopreservative, aliquoting and storing at -20°C.

  17. "Methodological Study on the Quantitative Detection of Tumor Marker CA72-4 Using a Microplate Chemiluminescent Enzyme Immunoassay." Blocking solution: 0.05 mol/L phosphate buffer (PBS, pH 7.4) containing 1% BSA and 0.1% Proclin-300.

  18. "Detection of Triiodothyronine in Human Serum Using a Microplate Chemiluminescent Immunoassay." Blocking solution: 0.05 mol/L phosphate buffer (PBS, pH 7.4) containing 0.5% BSA and 0.05% Proclin-300.

  19. "Research on a New Technique for the Measurement of Serum Creatinine Using a Two-Step Enzymatic Method." Reagent 1 and reagent 2 contained 200 μL of the preservative (Proclin-300) per liter of phosphate buffer solution.

  20. "Fitting and Optimization of S-shaped Standard Curve for Serum Hepatitis B Virus Large Protein Concentration." The HBV-LP standards were diluted in a 20 mmol/L PBS buffer solution with a pH of 7.4, containing 0.1% Proclin-300 and 4% bovine serum albumin (BSA), to different mass concentrations.

  21. "One-Step Chemiluminescent Immunoassay for the Detection of Thyroid-Stimulating Hormone in Dried Blood Spots." NP40s was optimized with the addition of 0.5 g cellulose, 0.5 g cyclodextrin, and 1 g Proclin-300 in 500 ml of bovine serum. The HRP-labeled mouse anti-human TSH monoclonal antibody was labeled using a conventional periodate oxidation method, and it was diluted with fetal bovine serum containing 1‰ Proclin-300 and 1% casein.

  22. "Application of a Biological Preservative in the Water Extract of Vomit Toxin." Different water extract matrices contained Proclin-300 at concentrations of 0.02% to 0.10%. They were placed at (28±1)°C (fungus test) and (37±1)°C (bacteria test) for 21 days. After 7 days, no microbial growth was observed, indicating that the preservative at 0.02% or higher concentrations had a bactericidal effect on the five indicator strains. The vomit toxin content did not show significant changes (P > 0.05).

  23. "Establishment and Evaluation of a Universal Circulating Immune Complex Antibody Dissociation Technique." After adding 0.02% Proclin-300 biological preservative, the solution was aliquoted and stored at 4°C as a positive control. The dissociation solution for CIC antibodies was prepared by mixing with equal amounts of different pH solutions and adjusting the pH to 7.3-7.5 while maintaining isotonic conditions with a corresponding concentration of Tris solution as the CIC antibody neutralizer. The osmotic pressure was adjusted with NaCl, and 0.02% Proclin-300 was added as a preservative. The dissociation solution for CIC antibodies contained a buffer solution of 0.06 mol/L Gly-HCl with pH 1.80, 0.085 mol/L NaCl, 0.02% Proclin-300 biological preservative. The CIC antibody neutralizer solution contained a water solution with 0.138 mol/L NaCl, 0.07 mol/L Tris, and 0.02% Proclin-300 biological preservative.

  24. "Establishment and Performance Evaluation of a Fluorescent Microsphere Immunoassay for Quantitative Measurement of Procalcitonin." Preparation of fluorescent microsphere labels: Finally, the solid precipitate was reconstituted in 1 mL of 0.01 mol/L phosphate buffer solution (pH 7.4), and 1 μL of Proclin-300 was added. The solution was stored at 4°C for later use.

  25. "Comparison of Quality Assurance Systems for Chinese and English Blood Screening Reagents." Therefore, some serum plates had to be primarily diluted with dilution fluid from high-titer samples. The dilution matrix used was Seracon II (SeraCare Life Sciences, Milford, MA), and the preservative (0.05% v/v) used was Proclin-300.


The antimicrobial agent, NeoCide PC-300, produced by Suzhou Genemill Biotechnology Co., Ltd., is fully equivalent to Proclin 300 and can be used as a substitute for Sigma Proclin 300. It is also exported to the United States and Germany. Some major IVD companies in China, including well-known publicly listed companies, are using the NeoCide series of antimicrobial agents. The price advantage is also evident. If you need assistance, our company can provide technical support and guidance!

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