Bronidox-L from Genemill(Can perfectly replace BASF Bronidox-L)

Application：

NeoCide™ BRO-NIDOX-L preservative(Bronidox-L) is designed for controlling microorganisms in biological media for in vitro diagnostic (IVD) products. The product contained 10% of 5-Bromo-5- Nitro-1,3-Dioxane in propylene glycol solution, colorless or light-yellow transparent liquid, with special bromine odor, which is a highly efficient biocide can be used for Hematology analysis, Chemistry analysis and Immunoassay analysis reagent as preservative, which is applied to all kinds of reagents including, controls, calibrators, diluent, system cleaning and buffer etc. Since the product has a wide range of the effect of inhibiting microbial growth, excellent compatibility and stability, low toxicity and biodegradability, NeoCide BRO-NIDOX-L preservative as one of option, can replace Sodium Azide, Thimerosal and Gentamycin. NeoCide BRO-NIDOX-L preservative can inhibit the growth of bacteria, fungi, yeasts in reagents for prolonged periods, thereby increasing a product’s shelf life and open vial stability. The water solubility permits easy incorporation into IVD reagents. Further, NeoCide BRO-NIDOX-L preservative does not affect the functionality of most enzyme- or antibody-linked reactions, and will not interfere with assay indicators, the material absorption peak at about 214 nm. The combination of NeoCide  BRO-NIDOX-L and PC-300/PC-950 is the most typical application scenario for CLIA buffers/concentrated buffers, with many successful cases in the AE platform and HRP/ALP platform.

Advantage：

NeoCide™ BRO-NIDOX-L(Bronidox-L) preservative has broad spectrum activity, excellent compatibility and stability, in the use of concentrations of low toxicity, became the ideal efficient antiseptic in vitro diagnostic (IVD) reagents. As one of important invention, Bromine related compounds had been developed as antimicrobial agents in the last seventy’s, especially Benzalkonium Bromide and Bronopol, these products are both biocide and surfactant with detergent function, as a disinfectant and preservative cosmetics production and extensive use in medical, although bacteriostatic effect is good, but because these compounds in aqueous solution can be precipitated, easy to change color and prone to bubbles, generally cannot be used as preservative in most of the in vitro diagnostic (IVD) reagent. The formation of 5-bromo-5-nitro-1,3-dioxane (BND) can not only improve the bacteriostatic ability, but also increase the stability under alkaline conditions, reduce discoloration and no longer release formaldehyde.  BRO-NIDOX-L which is 10% propylene glycol solution, easily applied into various kinds of in vitro diagnostic reagent, and can work with NeoCide ® PC series Preservatives like Isothiazolinone (like NeoCide PC-300/PC-950). Based on our extensive longstanding experience BRO-NIDOX-L is absolutely reliable anti-microbial biocide ideal for preserving liquid IVD formulations including reagents containing high protein, sugar and detergent concentrations that are particularly rich substrates prone to microbial deterioration. In recommended effective concentrations BRO-NIDOX-L is unrestrictedly compatible with all processes, buffers and reaction steps in ELISA, Blotting and related tests. It goes not interfere in coating/adsorption, analyte capturing (from different specimens including serum/plasma, urine, saliva, diluted excrements, etc.), detection and substrate. Its features and advantages include but are not limited to:

   o   Against bacteria, fungi and yeast all have better inhibition effect.

   o   Broad-spectrum and quickly eliminate the activity of microorganism and biofilm.

   o   Low concentration, low cost, no interference to the color of the reagent.

   o   Stable, at the same time worked as surfactant.

   o   Suitable for a wide range of pH, compatibility with enzymes and antibodies .

   o Environment friendly, biodegradability.

Clinical Hematology analysis system in the hospital could be contaminated easily as the reagent pickup systems or pipeline direct contact with blood, in the analysis process, which generated biofilm easily.  To use BRO-NIDOX-L as antibacterial agent against streptococcus pneumoniae in diluent and cleaning solution has special effect to extend the shelf life and open shelf-life stability. In the IA analysis, BRO-NIDOX-L Preservative as one of first choice applied to most of reagents.

Storage:

NeoCide™ BRO-NIDOX-L(Bronidox-L) Preservative storage temperature range of 15 to 40℃ for two years. In the process of storage and transportation, when the temperature is lower than 15℃, Bromonitrodioxane is stable and will not crystallize and precipitate out. Containers must be kept closed to avoid direct sunlight. Containers that have been opened must be carefully re-sealed and placed upright.

Mechanism of Action：

NeoCide™ BRO-NIDOX-L(Bronidox-L) Preservative is a kind of broad-spectrum antibacterial agent, main compound molecules electron-deficient oxidation of bromine atoms thiol oxidation bacteria enzyme, inhibit the activity of dehydrogenase causing irreversible damage to cell membrane, activation of bromine and cell membrane proteins at the same time, form a bromine compounds, nitrogen to interfere with the cell metabolism, bacteriostatic effect.

MIC：

NeoCide™ BRO-NIDOX-L(Bronidox-L) Preservative showed excellent anti-corrosion effect, its minimum inhibition concentration (MIC) as shown in the table below when the protein content of the reagents/solution is less than 10% of the formula. The product can be used for in vitro diagnostic reagents containing organic compounds, non-ionic or anionic surfactants and antigenic antibodies. The above components do not affect the antibacterial effect, nor do they produce antagonistic effects on each other. Compatible with most surfactants, but substances containing -SH groups, such as cysteine, can reduce the antibacterial activity. In vitro diagnostic reagents were generally added with a concentration of 0.1-0.5% (about 1-5L was added in 1000L reagents). 1L (equivalent to 0.1%) was added to 1000L of reagent, and the concentration was calculated as 1000ppm in BRO-NIDOX-L solution, and 100ppm in active substance (A.I.).