Application Scheme of Preservatives for Hematology (Blood Cell) Reagents - NeoCide Series Preservatives
You are here: Home » News » Application Scheme of Preservatives for Hematology (Blood Cell) Reagents - NeoCide Series Preservatives

Application Scheme of Preservatives for Hematology (Blood Cell) Reagents - NeoCide Series Preservatives

Views: 1     Author: Site Editor     Publish Time: 2024-02-29      Origin: Site

facebook sharing button
twitter sharing button
line sharing button
wechat sharing button
linkedin sharing button
pinterest sharing button
whatsapp sharing button
sharethis sharing button

The diluent, buffer solution, and hemolytic agent used in blood cell analysis can affect the detection of hemoglobin, red blood cells, platelets, and other components if the preservatives used are not appropriate. Based on users' practical experience, we recommend adding them at the beginning of the production process. They can be used in combination with other antimicrobial agents to form a dual or triple antimicrobial system. We recommend using Nuosha PC-150 in conjunction with BND-10 and GML-2 to achieve a synergistic effect. The recommended dosages are as follows:

NeoCide Preservatives

Cleaning solution

Dilution solution Three-part differential hemolytic agent Five-part differential reagent combination Staining solution reagent combination
Solution 1Solution 2
PC-150 0.1% 0.1% 0.2% 0.1% 0.1% 0.1%
GML-20.2% 0.1%-- 0.1% 0.1% 0.1%
BND-10 0.3% 0.1% 0.1%------

Note 1: Alkaline/strong alkaline (pH > 8.5) cleaning solutions, hemolytic agents, and blood cell reducing agents are not recommended to use PC-150. You can use an equivalent concentration (amount) of NeoCide BIT-10 (Product Code: GB01030) or NeoCide PC-950 (GB01009) as substitutes for PC-150.

Note 2: There are limited options for selecting antimicrobial agents for blood cell analysis reagents. Sodium azide is a traditional antimicrobial agent, but due to its toxicity and management costs, PC-150, PC-300, PC-950, or other antimicrobial combinations are generally used as substitutes. Integra-44 (Sodium N-hydroxymethylglycinate), Sodium Omadine, Bronopol, as they affect the degree of red blood cell lysis or interfere with hemoglobin measurement, are generally not used as antimicrobial agents in blood cell analysis reagents. Myacide SP (Dichlorobenzyl Alcohol), LiquiPar (Phenoxyethanol/Polymers), or IPBC (Iodopropynyl Butylcarbamate) are insoluble or prone to precipitation and are generally not used as antimicrobial agents.

Note 3: Hemolytic agents formulated with potassium cyanide do not require the addition of additional antimicrobial agents. Potassium cyanide itself can inhibit the respiratory enzyme system, cytochromes, cytochrome oxidase, catalase, and peroxidase in certain bacteria. By binding with iron porphyrin as a co-factor, potassium cyanide can deactivate these enzymes, thereby inhibiting bacterial growth.

Related Products

content is empty!

Specialized in developing, producing, and selling raw materials for IVD reagents.
    Address:No. 415 Changyang Street, Suzhou, Jiangsu, China




Copyright © 2023 Suzhou Genemill Biotechnology Co., Ltd. All rights reserved. Sitemap | Support By Leadong