Views: 2 Author: Site Editor Publish Time: 2024-02-29 Origin: Site
The diluent, buffer solution, and hemolytic agent used in blood cell analysis can affect the detection of hemoglobin, red blood cells, platelets, and other components if the preservatives used are not appropriate. Based on users' practical experience, we recommend adding them at the beginning of the production process. They can be used in combination with other antimicrobial agents to form a dual or triple antimicrobial system. We recommend using Nuosha PC-150 in conjunction with BND-10 and GML-2 to achieve a synergistic effect. The recommended dosages are as follows:
| NeoCide Preservatives | Cleaning solution (pH<8.5) | Dilution solution | Three-part differential hemolytic agent | Five-part differential reagent combination | Staining solution reagent combination | |
| Solution 1 | Solution 2 | |||||
| PC-150 | 0.1% | 0.1% | 0.2% | 0.1% | 0.1% | 0.1% |
| GML-2 | 0.2% | 0.1% | -- | 0.1% | 0.1% | 0.1% |
| BND-10 | 0.3% | 0.1% | 0.1% | -- | -- | -- |
Note 1: Alkaline/strong alkaline (pH > 8.5) cleaning solutions, hemolytic agents, and blood cell reducing agents are not recommended to use PC-150. You can use an equivalent concentration (amount) of NeoCide BIT-10 (Product Code: GB01030) or NeoCide PC-950 (GB01009) as substitutes for PC-150.
Note 2: There are limited options for selecting antimicrobial agents for blood cell analysis reagents. Sodium azide is a traditional antimicrobial agent, but due to its toxicity and management costs, PC-150, PC-300, PC-950, or other antimicrobial combinations are generally used as substitutes. Integra-44 (Sodium N-hydroxymethylglycinate), Sodium Omadine, Bronopol, as they affect the degree of red blood cell lysis or interfere with hemoglobin measurement, are generally not used as antimicrobial agents in blood cell analysis reagents. Myacide SP (Dichlorobenzyl Alcohol), LiquiPar (Phenoxyethanol/Polymers), or IPBC (Iodopropynyl Butylcarbamate) are insoluble or prone to precipitation and are generally not used as antimicrobial agents.
Note 3: Hemolytic agents formulated with potassium cyanide do not require the addition of additional antimicrobial agents. Potassium cyanide itself can inhibit the respiratory enzyme system, cytochromes, cytochrome oxidase, catalase, and peroxidase in certain bacteria. By binding with iron porphyrin as a co-factor, potassium cyanide can deactivate these enzymes, thereby inhibiting bacterial growth.